Depending upon the complexity of the assay, a single multiplex reaction can contain up to 40 markers. We only need to provide to the software the accession number on FASTA format. Using Assay Design Suite (ADS) software from Agena, we can design the PCR and iPLEX extension primers for each SNP or somatic mutation. The first step to perform our experiment is to design it. Here we will explain you more in details. The results report is obtained in less than one working day and with only 30 minutes of pipetting. That is why Assays by Agena allow us to analyze a combination of SNPs on 96- or 384-wells.įurthermore, this technology allows us to analyze at least 40 SNPs per well.Īnother of the main advantages of the use of MassARRAY technology is its workflow. MassARRAY technology is focused to provide solutions fastly and concisely. However, these candidate markers require complementary studies to validate them as a molecular diagnostic marker. Next generation sequencing ( NGS) and in general all genome association studies have stirred up the field of Genetics, facilitating the discovery of new genetic markers and contributing significantly to personalized medicine. This combination gives precision, fast, and cost-effective analysis from hundreds of genotypes per day. SNP genotyping on the MassARRAY System combines the robust multiplexed primer extension chemistry with highly sensitive mass spectrometry. With as little as 5 ng of DNA we can start our experiment. This starting material can be: blood, plasma, buccal swabs, and even compromised formalin-fixed, paraffin-embedded (FFPE) samples. MassArray, a technology able to analyze hundreds of mutations on a hundred of samplesĪgena allows us to analyze a variety of biomarkers from a full spectrum of biological samples. Is the acquisition of the raw data and its interpretation complicated?.The first step: to design our experiment.MassArray, a technology able to analyze hundreds of mutations on a hundred of samples.To sum up, Agena bioscience offers us an efficient, scalable, and cost-effective SNP genotyping. Research fields like routine genetic analysis of SNPs, linkage studies and validation of genome-wide association studies took advantage of this technology. It does not store any personal data.Since its introduction in 2000, the MassARRAY system (previously named Sequenom) has been widely adopted for a variety of genomic applications. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other. The cookies is used to store the user consent for the cookies in the category "Necessary". The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". Set by the GDPR Cookie Consent plugin, this cookie is used to record the user consent for the cookies in the "Advertisement" category. These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. They register anonymous statistical data on for example how many times the video is displayed and what settings are used for playback.No sensitive data is collected unless you log in to your google account, in that case your choices are linked with your account, for example if you click “like” on a video. These cookies are set via embedded youtube-videos. Some of the data that are collected include the number of visitors, their source, and the pages they visit anonymously.ġ6 years 3 months 22 days 12 hours 21 minutes Installed by Google Analytics, _gid cookie stores information on how visitors use a website, while also creating an analytics report of the website's performance. This cookie is set by Google and is used to distinguish users. The cookie stores information anonymously and assigns a randomly generated number to recognize unique visitors. The _ga cookie, installed by Google Analytics, calculates visitor, session and campaign data and also keeps track of site usage for the site's analytics report. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc. Analytical cookies are used to understand how visitors interact with the website.
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